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Friday, July 17, 2020 | History

2 edition of Histological evaluation of different methods for preservation of arterial grafts found in the catalog.

Histological evaluation of different methods for preservation of arterial grafts

Bengt E. Gustafsson

Histological evaluation of different methods for preservation of arterial grafts

by Bengt E. Gustafsson

  • 251 Want to read
  • 6 Currently reading

Published by Gleerup in Lund .
Written in English

    Subjects:
  • Arterial grafts -- Preservation.

  • Edition Notes

    Bibliography: p. 10.

    Other titlesArterial grafts.
    Statementby Bengt E. Gustafsson and Lars G. Hallén.
    SeriesLunds universitets årsskrift, n.f., avd. 2,, bd. 51, nr. 4, Kungl. Fysiografiska sällskapets handlingar, n.f.,, bd. 66, nr. 4
    ContributionsHallen, Lars Gunnar, 1914- joint author.
    Classifications
    LC ClassificationsAS284 .L82 bd. 51, nr. 4
    The Physical Object
    Pagination10 p.
    Number of Pages10
    ID Numbers
    Open LibraryOL211784M
    LC Control Numbera 57000193
    OCLC/WorldCa25186959

      Improved preservation and microcirculation with POLYSOL after transplantation in a porcine kidney autotransplantation model The most widely used preservation method for kidney grafts is cold static storage (CS) using the University of Wisconsin (UW) solution. prior to removal of the grafts for histological :// The terminology of bone transplantation is complicated by the various histologic types of bone grafts. A graft moved from one site to another within the same individual is an autograft; the

    Download Citation | Arterial Grafting for Coronary Artery Bypass Surgery | The second edition of this book provides updated knowledge about the biological characteristics and clinical use of   Kidney transplantation following uncontrolled donation after circulatory death (uDCD) presents a high risk of delayed graft function due to prolonged warm ischemia time. In order to minimise the effects of ischemia/reperfusion injury during warm ischemia, normothermic recirculation recently replaced in situ perfusion prior to implantation in several ://

      Static cold storage is the most prevalent method for preservation of renal grafts for transplantation (14, 17). The protective effects of various preservation solutions have been studied extensively in both clinical trials (11, 15) and several experimental animal models (5, 12, 21). However, few studies compared the preservation solutions in Patients who received single internal mammary artery and individual vein grafts 1 – 4 by on-pump bypass were included (full study protocol describing recruitment, clinical and health economics data collection, method of histological scoring, and standard techniques included as Methods and diagrammatic overview provided in Figure I in the


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Histological evaluation of different methods for preservation of arterial grafts by Bengt E. Gustafsson Download PDF EPUB FB2

Get this from a library. Histological evaluation of different methods for preservation of arterial grafts. [Bengt E Gustafsson; Lars Gunnar Hallen] The saphenous vein (SV) is limited in its use as a bypass conduit in coronary artery bypass grafting (CABG) when compared with arterial grafts because of structural and functional differences between veins and arteries.

1 Lower long-term graft patency rates and worse clinical outcomes have been reported after CABG performed with SV grafts compared with CABG performed with arterial grafts,   An evaluation by ex vivo isolated liver reperfusion indicated that the early graft function was significantly different between Group 1 (CS) and Groups 3 (SDP) and 2 (SBP).

Preserving small split-liver grafts using oxygenated HMP may be much more effective than other techniques and positively affect the prognosis of split-liver transplantation Preservation of endothelium was generally good in the cryopreserved allografts both with and without antibiotic pretreatment.

In general, the effects of cryopreservation, cyclosporine, and antibiotics ameliorated the effects of venous allografting into an arterial position. (J T horac C ardiovasc S urg ;—62) Histological analysis is the gold standard for tissue examination, either for research or diagnostic purposes, for both qualitative and quantitative measure.

It is used to assess the inflammation or healing stage and to monitor the presence and distribution of degradation products that dissolved into the surrounding :// Methods: This is a prospective experimental study. Fifteen diabetic and 15 non-diabetic patients were included to the study.

A total number of 96 graft samples were collected; 16 graft samples for each graft type from both diabetic and non-diabetic ://   Comparing three different preservation methods – formalin fixation (according to Platzer et al.

), Thiel fixation (according to Thiel, ), and alcohol–glycerine fixation (96% ethanol, 3% glycerine and 1% phenol)] – on the elastic and postyield mechanical properties of cortical bone with fresh-frozen specimens, the bone mineral Angioscopic evaluation of thick-walled (n = 10) and varicose veins (n = 2) was unreliable, inasmuch as all of these vein remnants were normal by angioscopic criteria.A single calcified vein (n = 1) was erroneously judged to be normal angioscopic appearance of 24 saphenous vein remnants was “abnormal,” and all of these segments were found to be abnormal histologically Comparing three different preservation methods – formalin fixation (according to Platzer et al.

), Thiel fixation (according to Thiel, ), and alcohol–glycerine fixation (96% ethanol, 3% glycerine and 1% phenol)] – on the elastic and postyield mechanical properties of cortical bone with fresh‐frozen specimens, the bone mineral Liver Grafts Procured From Donors After Circulatory Death Have No Increased Risk of Microthrombi Formation Cornelia J.

Verhoeven,1 Tiarah C. Simon,1 Jeroen de Jonge,1 Michael Doukas,2 Katharina Biermann,2 Herold J. Metselaar,3 Jan N. Ijzermans,1 and Wojciech G. Polak1 1Division of Hepatopancreatobiliary and Transplantation Surgery, Departments of Surgery, 2Pathology, and   1.

Introduction. Small diameter vascular grafts ( Although arterial grafts retain mechanical and functional similarity to coronary arteries, their application is hampered by vasospasm-related occlusion and vascular stenosis.

21 Certain studies suggested that the advantages of the application of IMA in comparison with other arterial grafts are related to its fewer smooth muscle cells, vast   The aim of the present study was to address histological changes in porcine ECM constitution induced by two different cell extraction methods; a non-ionic detergent Triton X and an enzymatic Trypsin decellularisation method.

Furthermore, the potential of seeded arterial endothelial cells to produce extracellular matrix components was :// Therefore, as a reliable reference method, histological evaluation and characterization of the morphological changes of the vascula r grafts before and during the cold storage period is ://   Autologous or synthetic vascular grafts are used routinely for providing access in hemodialysis or for arterial bypass in patients with cardiovascular disease.

However, some patients either lack suitable autologous tissue or cannot receive synthetic grafts. Such patients could benefit from a vascular graft produced by tissue engineering.

Here, we engineer vascular grafts using human allogeneic Methods: The decellularization is obtained through the use of water and SDS (0,3 %), after freezing at degrees, is the best alternative of different physical and/or chemical mechanisms to obtained from histological assessments were per-formed with Fisher’s exact test.

Morphometric data were analyzed with the Mann-Whitney U test. For the analysis of histological and morphometric data in a 3-group comparison of the ITBL, non-ITBL, and CRC groups at times 0 and 1, the chi-square test (exact method) and the Kruskall-Wallis test The animals were observed for 4 weeks after transplantation and then sacrificed.

Histologic evaluation of the tracheal allografts was routinely done using specimens from open biopsies. RESULTS: The overall length of tracheal grafts was +/- cm, and this variable was not significantly different between the four groups.

Graft exocrine Histology and Immunohistochemistry Evaluation. Preservation at 4°C under static conditions for 7 days with UW without HPL resulted in evidence of cell necrosis in the media by H&E staining (data not shown). For static and flow-preserved veins in UW with HPL for 7 days, the vascular structure assessed by H&E and Movat staining is shown in Due to poor vessel quality in patients with cardiovascular diseases, there has been an increased demand for small-diameter tissue-engineered blood vessels that can be used as replacement grafts in bypass surgery.

Decellularization techniques to minimize cellular inflammation have been applied in tissue engineering research for the development of small-diameter vascular ://.

There is a recent debate on the "transplantability" of ECD (Expanded Criteria Donors) kidneys and the selection criteria used to allocate them to single or double transplantation.

Remuzzi et al. have defined a protocol incorporating pre-transplant donor biopsy to guide the use of older donor organs. They allocated organs as single or double transplants on the basis of histological ://The development of novel biodegradable vascular grafts of a small diameter (arterial replacement.

Here we performed a pre-clinical study of new small-caliber biodegradable vascular grafts using a sheep model of carotid artery implantation.

The 4 mm diameter vascular grafts were manufactured using a mix of polyhydroxybutyrate/valerate and Post-implant evaluation of the anastomotic mechanical and geometrical coupling between human native arteries and arterial cryografts implanted in lower-limb Mechanical, histological and